ABSTRACT
Background and Purpose: Cytokines are among the many immune system factors involved in diabetes pathogenesis. The level of cytokines expression such as IFN- delta is varying in individuals and societies. Due to the fact that diabetes nephropathies are known as inflammatory disorders and the role of cytokines especially IFN- delta in the establishment of inflammations is well understood, the present study was aimed to examine serum level of IFN-delta in type 2 diabetes patients with nephropathy complications
Material and methods: In this cross sectional descriptive study, serum samples were obtained from 100 type 2 diabetes patients with nephropathy and 100 healthy controls for the analysis of IFN- delta serum level [eBiosense, ESP]. Consent forms were also filled out by patients and healthy controls according to the rules and regulations of Zahedan Islamic Azad University Ethical Committee. The obtained data and questionnaires were analyzed in SPSS using ANOVA; p<0.05 was considered as significant
Results: The mean serum level of IFN- delta was 16.09 +/- 7.74 pg/ml and 4.03 +/- 2.00 pg/ml in nephropathic patients and healthy controls, respectively. Statistical analysis of the data showed that the difference in the IFN- delta level was not significant in nephropathic patients and controls
Conclusion: Based on the non-significant differences between the two groups, it seems that there is no association between the level of serum IFN- delta and nephropathy in type 2 diabetics
ABSTRACT
Occult hepatitis B infection [OBI] is a form of hepatitis, which in despite of absence of detectable HBsAg, HBV-DNA is present in peripheral blood of patients. Evaluation the relationship between alleles of+1188 in region of IL-12 with serum level of cytokine in patients with occult HBV infection. In this study, the plasma samples of 3700 blood donors were tested for HBsAg and anti-HBc by ELISA. The HBsAg negative ve and anti-HBc positive samples were selected and screened for HBV-DNA by PCR. HBV-DNA positive samples assigned as OBI cases and PCR-SSP and ELISA were performed to examine the polymorphisms in region of [+1188 and serum level of IL-12] respectively. The results showed that there is a significant difference in CC allele of+1188 region of IL-12 in two groups and no difference in the other evaluated genes. There is not any significant difference in serum level of IL-12 between OBI patients and controls. Our results also showed that there isn't any significant statistically relation between alleles of+ 1188 region of IL-12 with serum level of cytokine. According to the results of this study it could be concluded that OBI patients unable to produce enough quantity of IL-12 and it may be related to different IL-12 gene. CC allele was associated with OBI, hence, it seems that +1188 region of IL-12 gene has an important role in expression of IL-12 gene. Evaluation of relation between polymorphisms in +1188 region of IL-12 gene and its expression. In vitro and under mitogene affect can useful because no association was seen between serum level of IL-12 and alleles of this region